PL-002 AGTR1 polymorphism is associated with elite endurance athletes: A functional study

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Abstract

Objective To explore the association between the polymorphism of angiotensin II receptor type 1 gene(AGTR1) and elite endurance athlete performance and the mechanism of how the polymorphism works.

Methods (1) Polymorphism of AGTR1 rs5182 between 122 elite Chinese endurance athletes and 222 controls were analyzed by MALDI-TOF-MS. (2) Aerobic capacity of 79 elite Chinese endurance athletes such as VC/FEV1/MVV/ VO2AT/ HRAT/ VAT/ VO₂max/ HRmax/ VVO₂max were measured and association between rs5182 polymorphism and the performance was analyzed. (3) PcDNA3.1-AGTR1 -T and pcDNA3.1-AGTR1-C plasmid were build and the plasmids was transfected into mammalian 293T cells. mRNA levels were detected after 48 hours. Statistical analysis was performed using SPSS software version 15.0. Values of P < 0.05 were considered statistically significant. Continuous data were expressed as mean ± SD, while categorical data were expressed as frequencies. Genotype distribution and allele frequencies between athletes and control subjects were compared using χ2 tests. Aerobic performance data was analyzed with One-Way ANOVA if it conformed to normal distribution and homogeneity of variance otherwise Non-parametric test of independent sample was used.

Results (1) Genotype frequencies of AGTR1 rs5182 are significant differences between the athletes and control subjects (p = 0.040), the Word-Class athletes and control subjects (p = 0.018), 5km athletes and control subjects (p =0.015), 10km athletes and control subjects (p = 0.026), male athletes and male controls(p=0.045). (2) Association is found between Genotype distribution and MV(L/min) though others not (Genotype: MV; CC: 122.514±6.767; CT:117.187±17.961; TT:119.688±20.226, p=0.047). (3) Transiently transfectedpcDNA3.1-AGTR1-T and pcDNA3.1-AGTR1-C plasmids into 293T cells successfully. The differences of mRNA levels between the groups were not significant (p = 0.991).

Conclusions  AGTR1 gene rs5182 could be a candidate genetic mark of selection elite endurance athletes in Han Population from Northern China, but this polymorphism does not affect AT1R protein function through changing its mRNA level.