PO-197 Effects of aerobic exercise training on F13A-mediated energy metabolism in mice

There is no full paper associated with this abstract

Abstract

Objective Apelin, an adipokine and also a myokine, is involved in energy metabolism. F13A is an analog of apelin-13. In this study, we aim to investigate the effect of aerobic exercise on F13A-mediated energy metabolism in mice.

Methods 20 C57BL/6J wild mice were randomly divided into 4 groups(n:5), namely saline control group(SC), saline exercise group(SE), F13A control group(FC), and F13A exercise group(FE). Mice were intraperitoneally injected with F13A(0.2 μmol/kg/day) or saline(15 μl/kg/day). Mice in the exercise group underwent 60 min/day treadmill running at a speed of 15 m/min with a slope of 5 º. After 2 weeks, the maximal oxygen uptake was measured and the running speed was adjusted to 20 m/min. The treadmill running continued 4 weeks. The mice were individually housed in a Comprehensive Lab Animal Monitoring System(Columbus Instruments, Columbus, OH, USA) between the 3^rd and 4^th week of training with free access to food and water. O₂ consumption(V_O2), CO₂ production(V_CO2) and respiratory exchange ratio(RER) during a 24-h period were measured after 24h of acclimatization. Glucose oxidation(in g/min/kg^0.75=[(4.545×V_CO2)−(3.205×V_O2)]/1000), and lipid oxidation(in g/min/kg^0.75=[1.672×(V_O2−V_CO2)]/1000) were calculated.

Results F13A alone increased glucose oxidation(P<0.01, vs SC group). Exercise plus F13A caused a significant decline in RER(P<0.01 vs FC and P<0.05 vs SE group), glucose oxidation(P<0.001 vs FC and P<0.05 vs SE group), whereas it increased lipid oxidation(P<0.05 in comparison with FC group). Exercise alone has no in fluence on 4 groups.

Conclusions These findings suggest that 4 weeks aerobic exercise can regulate F13A reduce RER in mice, with a decrease of glucose oxidation and an increase of lipid oxidation in vivo.